FIGURE

FIGURE 1

ID

ZDB-FIG-240417-29

Publication

Liu et al., 2024 - Plexin B3 guides axons to cross the midline in vivo

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FIGURE 1

Plexin B3 is expressed in retinal ganglion cells when retinal axons are crossing the midline. In situ hybridization was performed on Tg[Isl2b:GFP] transgenic embryos at 34–36 hpf (hour post fertilization), in which the reporter protein GFP is expressed specifically in retinal ganglion cells and can also label retinal axons. (A) Fluorescent in situ hybridization using complementary RNA probe reveals that Plexin B3 is strongly expressed in retina at 34 hpf. (B) Tg[Isl2b:GFP] transgenic labeling demonstrates that Plexin B3 is strongly expressed in retinal ganglion cell layer (square brackets) when pioneer retinal axons are starting to cross the midline and form the chiasm. (C,D) Plexin B3 is expressed in retinal axon ganglion cell layer at 36 hpf when many retinal axons cross the midline. (E,F) A zoomed in and single plane of confocal image demonstrates that Plexin B3 is expressed in retinal ganglion cell layer. All images are ventral view, rostral to the top. Scale bar, 100 μm.

Expression Data

Genes:	GFP plxnb3
Fish:	zc7Tg (AB)
Anatomical Terms:	retina retina axon retinal ganglion cell retinal ganglion cell layer
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.