FIGURE

Figure 4

ID

ZDB-FIG-240331-4

Publication

Zhou et al., 2024 - Systematic Identification of Long Noncoding RNAs during Three Key Organogenesis Stages in Zebrafish

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Figure 4

The effects of lncRNA gas5 knockout. (a) The expression level of lncRNA gas5 knockout embryos, detected by qPCR. Data derived from three independent experiments are shown as mean ± SEM. **** indicates p < 0.0001. (b) The expression level of lncRNA gas5 knockout embryos, detected by in situ hybridization. NI represents no injection, control represents only injection of Cas9 protein, Cas9 represents co-injection of sgRNA and Cas9 protein. Microscope’s magnification: 45 × (c–h) The effects of knockout on embryonic development. Microscope’s magnification: 45 ×. #: enlargement of yolk extension. *: small or absent eyes, arrows indicate nonseparation of two otoliths.

Expression Data

Gene:	gas5
Fish:	AB + CRISPR10-gas5
Knockdown Reagent:	CRISPR10-gas5
Anatomical Terms:	brain eye spinal cord
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR10-gas5
Knockdown Reagent:	CRISPR10-gas5
Observed In:	brain embryo development eye spinal cord whole organism
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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