FIGURE

Fig. 3

ID

ZDB-FIG-240219-33

Publication

Spratt et al., 2023 - Multivalent insulin receptor activation using insulin-DNA origami nanostructures

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Fig. 3

Valency of insulin on NanoRods determines the residence time of IR binding.

a, Schematic of the SPR assay: biotinylated ECD-IR was immobilized onto a streptavidin-coated SPR surface followed by incubation with INS-DNA or insulin NanoRods. b, SPR traces showing the binding of INS-DNA and of the indicated insulin NanoRods to the ECD-IR. K_D was calculated from the curves obtained from INS-DNA, NR-2, NR-4 and NR-7. RU, resonance units. c, Residence time (t_1/2) of the insulin NanoRods and INS-DNA on ECD-IR, calculated from the SPR binding curves. The values shown are from one representative experiment out of two independent experiments.

Source data

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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