FIGURE

Fig. 3

ID

ZDB-FIG-240116-11

Publication

Yamamoto et al., 2024 - Trpv4-mediated apoptosis of Leydig cells induced by high temperature regulates sperm development and motility in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Leydig cells in Trpv4 KOs did not undergo apoptosis in high temperature.

a HE staining of testes. The upper panels show Trpv4^+/+ and the lower panels show Trpv4^−/−. Black line indicates areas of E3 spermatid. Red line indicates an abnormal cell population. Arrows indicate interstitial tissue. Interstitial tissue can be detected clearly in Trpv4^−/− after 7 and 14 days of temperature stimulation. b qRT-PCR analysis of Leydig cell marker genes in testes. Values are the mean ± SEM (n = 4). *p < 0.01 compared with the 29 °C testis. c In situ hybridization in testes of Isl3, which is a Leydig cell marker. d In situ hybridization of Hspa5. Black broken lines indicate interstitial cells and red broken lines indicate spermatocyte cells. e Localization of cleaved caspase 3. White broken lines indicate interstitial cells and red broken lines indicate spermatocyte cells. Scale bars are 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Commun Biol