FIGURE

Figure 4

ID

ZDB-FIG-240113-33

Publication

Sobah et al., 2023 - Contribution of Signal Transducer and Activator of Transcription 3 (STAT3) to Bone Development and Repair

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Figure 4

Effect of Stat3 mutations on bone formation. Representative images of Stat3 wildtype (WT: A,D,G,M,P), knockout (KO: B,E,H,N,Q), and transactivation domain truncation (ΔTAD: C,F,I,O,R) mutant embryos subjected to WISH at 50 hpf with runx2b (A–C) and at 3 dpf with col10a (D–F) or spp1 (G–I) in lateral view as indicated with black arrowheads, or at 8 dpf with calcein staining (lateral: M–O; ventral: P–R), with scale bars representing 200 μm. Quantification of area of staining for runx2b (J), col10a (K), and spp1 (L), showing individual values for each embryo and of specific bones (S,T), with mean, SEM, and statistical significance indicated (*** p < 0.001, ** p < 0.01, * p < 0.05, ns: not significant). Abbreviations: br1/2: branchiostegal ray 1/2; c: cleithrum; cb: ceratobranchial; ch: ceratohyal; d: dentary; o: opercula.

Expression Data

Genes:	col10a1a runx2b spp1
Fish:	WT stat3^mdu35/mdu35 stat3^mdu36/mdu36
Anatomical Terms:	osteoblast osteoprogenitor cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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