FIGURE

Fig. 4

ID

ZDB-FIG-240109-23

Publication

Chang et al., 2023 - Activation of Nrf2 by sulfuretin stimulates chondrocyte differentiation and increases bone lengths in zebrafish

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Fig. 4

Nrf2 is necessary for effects of sulfuretin on chondrocyte and bone growth in zebrafish. (A) Induction of chondrocyte genes by sulfuretin is blunted in Nrf2 knockdown cells. C3H10T1/2 cells were transfected with siRNA targeting Nrf2 (Nrf2 siRNA) and differentiated into chondrocytes for 12 days. Expression levels of Nrf2 targets (Hmox1 and Nqo1) and chondrocyte genes (Mmp3 and Mmp13) were measured by real-time PCR. Data are presented as mean ± SEM (n = 3). (B) Effects of sulfuretin and DMF on body lengths were compromised in nrf2 morpholino injected zebrafish. Sulfuretin (100 μM) or DMF (4 μM) was used to treat control morpholino or nrf2 morpholino injected zebrafish at 1 dpf for 4 days and their lengths were determined. Data are presented as mean ± SEM (n = 10). Statistical significance was determined by comparison with the control using Student’s t-test (ns, not significant; *P < 0.05; **P < 0.01).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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