PUBLICATION
Activation of Nrf2 by sulfuretin stimulates chondrocyte differentiation and increases bone lengths in zebrafish
- Authors
- Chang, S.H., Giong, H.K., Kim, D.Y., Kim, S., Oh, S., Yun, U.J., Lee, J.S., Park, K.W.
- ID
- ZDB-PUB-231002-116
- Date
- 2023
- Source
- BMB reports 56: 496501496-501 (Journal)
- Registered Authors
- Giong, Hoi-Khoanh, Lee, Jeong-Soo
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation
- Chondrocytes*
- Mammals
- NF-E2-Related Factor 2*
- Zebrafish
- PubMed
- 37748761
Citation
Chang, S.H., Giong, H.K., Kim, D.Y., Kim, S., Oh, S., Yun, U.J., Lee, J.S., Park, K.W. (2023) Activation of Nrf2 by sulfuretin stimulates chondrocyte differentiation and increases bone lengths in zebrafish. BMB reports. 56:496501496-501.
Abstract
Elongation of most bones occur at the growth plate through endochondral ossification in postnatal mammals. The maturation of chondrocyte is a crucial factor in longitudinal bone growth, which is regulated by a complex network of paracrine and endocrine signaling pathways. Here, we show that a phytochemical sulfuretin can stimulate hypertrophic chondrocyte differentiation in vitro and in vivo. We found that sulfuretin stabilized nuclear factor (erythroid-derived 2)-like 2 (Nrf2), stimulated its transcriptional activity, and induced expression of its target genes. Sulfuretin treatment resulted in an increase in body length of zebrafish larvae and induced the expression of chondrocyte markers. Consistently, a clinically available Nrf2 activator, dimethyl fumarate (DMF), induced the expression of hypertrophic chondrocyte markers and increased the body length of zebrafish. Importantly, we found that chondrocyte gene expression in cell culture and skeletal growth in zebrafish stimulated by sulfuretin were significantly abrogated by Nrf2 depletion, suggesting that such stimulatory effects of sulfuretin were dependent on Nrf2, at least in part. Taken together, these data show that sulfuretin has a potential use as supporting ingredients for enhancing bone growth. [BMB Reports 2023; 56(9): 496-501].
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping