Fig 5

Rag2:EGFP-CA-CaMKII; tp53 mutant fish develop acute lymphoblastic leukemia/lymphoma.

Hematoxylin and eosin stained sections of the (A-D) head kidney and (E-H) spleen of nine month old (A, E) wild type, (B, F) rag2:EGFP-CA-CaMKII transgenic, (C, G) tp53 mutant, and (D, H) rag2:EGFP-CA-CaMKII; tp53 mutant fish. (D, H) An increase in lymphoblasts is observed in the kidney and spleen of rag2:EGFP-CA-CaMKII; tp53 mutant fish. (I-L) Anti-EGFP immunohistochemistry indicates EGFP positive lymphocytes are increased in the (J) kidney marrow and (L) spleen of leukemic fish compared to (I, K) rag2:EGFP-CA-CaMKII wild type fish. Kidney marrow (KM) cytospins of (M) wild type, (N) rag2:EGFP-CA-CaMKII transgenic, (O) tp53 mutant, and (P) rag2:EGFP-CA-CaMKII; tp53 mutant were HEMA3 stained. (Q) Four cohorts of fish were assessed for survival during the first year. p<0.0001 comparing rag2:EGFP-CA-CaMKII; tp53 wild type fish to rag2:EGFP-CA-CaMKII; tp53 mutants and tp53 mutants to rag2:EGFP-CA-CaMKII; tp53 mutants for survival using the Log-rank (Mantel-Cox) test. A statistically significant reduction in survival of rag2:EGFP-CA-CaMKII; tp53 fish was evident, p<0.01. Scale bars: 40 μm in A and 10 μm in I and Q.