Fig. 6

fbox3-null zebrafish display higher resistance to SVCV infection in liver and spleen. (A–D) Induction of key antiviral genes was more significant in fbxo3-null ZFL compared with the WT ZFL infected with SVCV. (E–H) Induction of key antiviral genes was more significant in fbxo3-null zebrafish spleen compared with the WT zebrafish spleen infected with SVCV. (I–K) The virus replication number indicated by the expression of the G (I), N (J), and P (K) genes of SVCV, was lower in the fbxo3-null ZFL compared with the WT ZFL infected with SVCV. (L) Representative images of fbxo3-null zebrafish (2 mpf) and their WT siblings (2 mpf) i.p. injected with or without SVCV at 10 μl per individual for 48 h. Red arrow indicates hemorrhage. (M) fbxo3-null zebrafish (2 mpf) (n = 20) were more resistant to SVCV infection compared with the WT (2 mpf) (n = 20) based on the survival ratio. Data are presented as means ± SEM of three biological replicates. ***p < 0.001, ****p < 0.0001.