Clasp2 mRNA distribution in adult guppy testis, confocal microscopy. (a) Fluorescence in situ hybridization of clasp2 shows the labeling concentrated at the periphery of numerous spermatozeugmata. (b) High magnification of a portion of spermatozeugmata in a white rectangle is shown in panel a. (c) Fluorescence in situ hybridization of clasp2 is seen at the periphery of spermatozeugmata cysts. (d) DAPI staining marks all the cell nuclei of the testis. (e) The merge of fluorescence in situ hybridization of clasp2 and DAPI in adult guppy testis. Abbreviation: Sz; spermatozeugmata. Scale bars are 50 µ (a); 10 µ (b); and 30 µ (c–e).
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