Eupatilin and quercetin, the potential active ingredients of Aquilaria sinensis leaf tea, improve the sleep of zebrafish with light-induced sleep disturbance. (A) 3D chemical structure of eupatilin based on PubChem database. (B) Molecular docking of eupatilin with Cd40. (C–D) Sleep and waking activity traces (±SEM) of zebrafish larvae treated with eupatilin. The gray box indicates sleep disturbance time (300 lux light on 23:00–03:00) (n = 48). (E) Quantification of total sleep across day time (9:00–23:00), sleep disturbance time (23:00 to 03:00), and night without sleep disturbance time (03:00–09:00) on 6 dpf in eupatilin treated and control groups (n = 48). (F) Quantification of sleep bout on sleep disturbance time between control and eupatilin treated groups (n = 48). (G) 3D chemical structure of quercetin based on PubChem database. (H) Molecular docking of quercetin with Il1b. (I–J) Sleep and waking activity traces (±SEM) of zebrafish larvae treated with quercetin (n = 48). (K) Quantification of total sleep across day time (9:00–23:00), sleep disturbance time (23:00 to 03:00), and night without sleep disturbance time (03:00–09:00) in quercetin treated and control groups on 6 dpf (n = 48). (L) Quantification of sleep bout on sleep disturbance time between control and quercetin-treated groups (n = 48). Lowercase letters indicate significant differences (one-way ANOVA, Tukey’s post hoc test. Data are shown as mean ± standard deviations. p < 0.05).
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