Figure 4—figure supplement 1.

Changes in the main ciliary beating frequency varied from fish to fish in response to RF photoablation.

(A) The main beating frequencies of dorsal and ventral motile cilia after RF photoablation (mean ± standard deviation provided hereafter: 11.9 Hz ± 2.0 Hz) compared to those before RF photoablation (11.7 Hz ± 2.4 Hz), illustrating that cilia main beating frequency was on average not significantly affected by RF photoablation (N=76 cilia from 9 fish; paired two-tailed t-test: p > 0.3). (B1–B3) Select examples from (A), depicting fish whose response to RF photoablation significantly changed the main ciliary beating frequencies but in different ways. B1 and B3 illustrate an increase in main ciliary beating frequency after RF photoablation (B1: mean ± standard deviation provided hereafter: 11.9 Hz ± 2.5 Hz; B3: 12.5 Hz ± 1.4 Hz) from that before RF photoablation (B1: 10.9 Hz ± 2.5 Hz; N=12 cilia; paired two-tailed t-test: p < 0.05; B3: 11.3 Hz ± 2.3 Hz; N=8 cilia; paired two-tailed t-test: p < 0.05). B2 illustrates a decrease in main ciliary beating frequency after RF photoablation (11.6 Hz ± 2.2 Hz) from that before RF photoablation (12.6 Hz ± 1.9 Hz; N=11 cilia; paired two-tailed t-test: p < 0.01). * p < 0.05, ** p < 0.01.