Lineage tracing of CNCCs in PA1 and frontonasal prominence using sox10:Dendra2. (A) Photoconversion was performed in sox10:Dendra2 transgenic zebrafish. Photoconvertible fluorescent protein Dendra2 is photoactivated by UV light (405 nm) from green to red. Specific Dendra2-expressing cells were labeled irreversibly and traced. (B–D′″) Lineage tracing of the PA1 components: maxillary prominence (B, B′), mandibular prominence (C, C′), and frontonasal prominence (D, D′). Magnified view of the photoconverted each prominence was displayed in panel B′, C′, and D′. (B–B′″) The maxillary prominence was labeled at 24 hpf and differentiated into lateral part of the ethmoid plate (zebrafish palate) and the pterygoid process (ptp), which is the upper jaw at 72 hpf. (C–C′″) The mandibular prominence was labeled at 24 hpf and differentiated into Meckel’s cartilage of the lower jaw. (D–D′″) The frontonasal prominence was labeled at 24 hpf and differentiated into the medial part of the palate at 72 hpf. (E–E″) The schematic diagram of the lineage tracing of the PA1 and frontonasal prominence. Each color in the panel E represents the following: yellow indicates the photoconverted region of the maxillary prominence, orange indicates the photoconverted region of the mandibular prominence, and blue indicates the photoconverted region of the frontonasal prominence. ch, ceratohyal; e, eye; gc, gill cartilages; m, Meckel’s cartilage; mx, maxillary; p, palate; ptp, pterygoid process; tr, trabeculae. Scale bar: 100 µm.
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