Fig. 5

Larval behavior of hrh1^+/+ and hrh1^−/− fish. a Total distances moved of hrh1^+/+ and hrh1^−/− larvae at 6 dpf in an open field for 15 min. b The frequency of zebrafish entering the center zone of an open field. c The duration of zebrafish staying in the center zone of open field. d Dark flash responses of hrh1^+/+ and hrh1^−/− zebrafish. Each dark period lasted 2 min. Distance moved during every 30-s period is indicated on the Y axis. e Distance moved/s of hrh1^+/+ and hrh1^−/− in the first dark response period. f The index of the first dark response (distances moved in the dark (30 s) minus distances moved in the light (30 s)). a–fN (hrh1^+/+) = 17, N (hrh1^−/−) = 27. g Sleep-like behavior from 5 to 6 dpf of hrh1^+/+ and hrh1^−/− zebrafish. Distance covered during every 30-min period is indicated on the Y axis. N (hrh1^+/+) = 16, N (hrh1^−/−) = 14. h–i Mean values for maximum velocity during 10 acoustic/vibrational stimuli with a 20-s interstimulus interval (ISI) (h) and 30 acoustic/vibrational stimuli with 1 s ISI. N (hrh1^+/+) = 14, N (hrh1^−/−) = 15. Mean ± SEM, two‐way ANOVA