FIGURE
Fig. 3
- ID
- ZDB-FIG-230912-9
- Publication
- Zhang et al., 2023 - Knockout of miR-184 in zebrafish leads to ocular abnormalities by elevating p21 levels
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Fig. 3
Mir-184−/− zebrafish displayed a cataract phenotype. (A) The cataract phenotype was detected using a grid transmission assay in 6-month-old and 12-month-old miR-184−/− zebrafish. (B) The cataract phenotype of miR-184−/− zebrafish was observed with a split lamp microscope. (C) WT and miR-184−/− lenses were stained with phalloidin to label the F-actin of lens cytoskeleton at 2 mpf, 3 mpf, 6 mpf, and 12 mpf. The nuclei were stained with DAPI. Bar, 100 μm. (D) The water solubility, SDS-solubility, and SDS-insolubility of lens crystallin in 6-month-old WT and miR-184−/− lenses were analyzed by immunoblotting. β-actin was as the endogenous control. ns, nonspecific bands. (E) The quantitation of α-, β-, and γ-crystallin levels in (D). The data showed means ± SD.
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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