Fig. 3

Mir-184^−/− zebrafish displayed a cataract phenotype. (A) The cataract phenotype was detected using a grid transmission assay in 6-month-old and 12-month-old miR-184^−/− zebrafish. (B) The cataract phenotype of miR-184^−/− zebrafish was observed with a split lamp microscope. (C) WT and miR-184^−/− lenses were stained with phalloidin to label the F-actin of lens cytoskeleton at 2 mpf, 3 mpf, 6 mpf, and 12 mpf. The nuclei were stained with DAPI. Bar, 100 μm. (D) The water solubility, SDS-solubility, and SDS-insolubility of lens crystallin in 6-month-old WT and miR-184^−/− lenses were analyzed by immunoblotting. β-actin was as the endogenous control. ns, nonspecific bands. (E) The quantitation of α-, β-, and γ-crystallin levels in (D). The data showed means ± SD.