Figure 2

Phenotype analysis of morpholino mediated knockdown of Aicardi candidate genes in zebrafish embryos. (a) Examples of morphology of zebrafish embryos at 72 h post fertilization (hpf) either uninjected or injected with a control- or gene-targeted morpholino. Note the reduction in eye pigment in embryos injected with the tead1, slf1 or wnt8b morpholinos. (b) Zebrafish embryos were scored according to the classification system of Miesfeld et al. (2015) [58]. Class I: normal phenotype; Class II: normal eye pigmentation with mild body defects; Class III: reduced eye pigmentation with normal to mild body defects; Class IV; reduced eye pigmentation and severe body defects. (c) Observed morphant phenotypes after researcher-blinded injections of 240 zebrafish embryos per group. The number of viable embryos remaining after 72 hpf are denoted by (n). The amount of morpholino injected per group are indicated by (+).