Figure 5

Klf9^−/− mutants have larger livers than wildtype larvae. (a) Image of Alexafluor568-Streptavadin-stained larva showing how the liver was located and segmented to create a 3-D surface for estimating its volume in Imaris. (b) Measurements of liver volumes, combined from separate analyses performed blind by two different individuals. The two sets of measurements differed slightly but systematically, so were brought into register by mean-centering all measurements taken by each experimenter before integrating their data (see Methods and Supplemental Fig. S8). This allowed each individual measurement to be plotted as a quantity relative to the average (“0” on the y-axis). The green diamonds show the 95% confidence interval of the mean, which is indicated by the central horizontal green line; the other two horizontal green lines delimit the region within which the two means are not significantly different. Significance was calculated by t-test.