FIGURE

FIGURE 12

ID

ZDB-FIG-230731-161

Publication

Farre et al., 2023 - Long wavelength-sensing cones of zebrafish retina exhibit multiple layers of transcriptional heterogeneity

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FIGURE 12

Expression of si:busm1 in larval zebrafish. (A–D) Projections of representative whole, imaged eyes. (E–F”’) Single z slices obtained from the same preparations. Note reduced expression domain of lws2 (cyan), and expanded expression domains of lws1 (magenta) in T3-treated (B,D) vs. controls (A,C); the si:busm1 (yellow) expression domain appears greatly reduced in the treated condition (D). (E’–E”’) Enlarged images of region within box in panel (E). (E’) All imaging channels merged. (E”)si:busm1(E”’)lws2(E””)lws1(F’–F””) Enlarged images of region within box in panel (F). (F’) All imaging channels merged. (F”)si:busm1(F”’)lws2(F””)lws1(G–J) Projections of whole embryo heads. (K) qPCR quantification of gngt2a transcript abundance in pooled samples of whole larvae, n = 5 biological replicates, p = 0.244. (L) 3D fluorescence intensity quantification, n = 5 (DMSO), 4 (T3), p = 0.056 (t-test). D, dorsal, T, temporal. n.s., not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.