Expression of vax1 and vax2 in adult zebrafish retina. (A–C”’)vax1. (D–F”’)vax2. (A) Expression of lws1 (magenta) and lws2 (cyan) in a representative whole retina. (A’)vax1 expression (yellow) in the same preparation, showing expression restricted to the lws1-expressing domain. (B) 40x image of lws1, lws2, and vax1 expression, in a region of lws1 to lws2 transition; orientation of panels (B,B’) are such that dorsal is to the right. (B’) 40x image of vax1 alone, showing transition to vax1 domain is less abrupt than the transition to the lws1 domain. (C–C”’) Resliced orthogonal projections of panel (B). (C) All imaging channels merged. (C’) DAPI and vax1. (C”) DAPI and lws1.(C”’) DAPI and lws2.(D) Expression of lws1 (magenta) and lws2 (cyan) in a representative whole retina. (D’)vax2 expression (yellow) in the same preparation, showing expression restricted to the lws1-expressing domain. (E) 40x image of lws1, lws2, and vax2 expression, in a region of lws1 to lws2 transition. (E’) 40x image of vax2 alone in the same region, showing transition to vax1 domain is less abrupt than the transition to the lws1 domain. (F–F”’) Resliced orthogonal projections of panel (E). (F) All imaging channels merged. (F’) DAPI and nr2f2. (F”) DAPI and lws1.(F”’) DAPI and lws2. D, dorsal. Sample size = 2.
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