Figure 8

Characterization of changes in dendrite-like neurites during adult zebrafish RGC axonal regeneration. Time-lapse live imaging in sparesely labeled, mixed adult zebrafish retinal cultures seeded in a SOC450 MFD enables characterization of dendritic remodeling during axonal regeneration of adult zebrafish RGCs. Representative confocal still overview pictures of isolated gap43 RGCs (top panels) and detailed images of the dendritic area (bottom panels, with colored arrowheads indicating the same dendrite-like neurites over time and * indicating the axotomized axon) in a mixed Tg(Tru.gap43:GFP)^mil1/wild type (gap43/WT) retinal cell culture illustrate both axonal regeneration and temporary dendritic remodeling (in length and relative position) after axotomy at DIV 3 (0–3 hpi). These first evaluations indicate the potential of this sparsely-labeled setup to visualize and characterize dendritic changes during axonal regeneration of adult zebrafish RGCs. Scale bars: 100 μm (overview pictures); 50 μm (magnified inserts). AC, axonal compartment; DIV, days in vitro; MFD, microfluidic device; RGC, retinal ganglion cell; SDC, somatodendrtic compartment.