Figure 1

Rgp1 is required for zebrafish craniofacial skeletal development. (A) Zebrafish rgp1 intron/exon map showing locations of g1 and g3 gRNA target sites. (B) Sequences from three different alleles isolated from the use of g1 and g3 gRNAs. (C) Predicted peptide lengths for alleles shown in (B). (D) Live imaging of 5 dpf WT and rgp1^-/- larvae from ventral and lateral view showing jaw protrusions (black arrowhead). (E) Alcian blue staining and imaging of 5 dpf WT and rgp1^-/- cartilage in the ventral craniofacial head skeleton. The Meckel’s cartilage (red), and ceratohyal (yellow, dashed) are marked. (F) Quantification of larva head length. (G) Quantification of Meckel’s cartilage length. (H) Quantification of ceratohyal cartilage angle. (I) Schematic for zebrafish rgp1 mRNA injection and rescue experiment. (J) Lateral images of larvae showing jaw protrusion, partial protrusion, and no protrusion phenotypes (black arrowheads). (K) Quantification of jaw phenotype percentages in groups of injected and non-injected embryos. Significance is presented by ****p<0.0001.