Figure 4
- ID
- ZDB-FIG-221230-38
- Publication
- Bello-Rojas et al., 2022 - Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits
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(A) Schematic of larval zebrafish whole-cell paired recording (sister V2a in red and sister V2b in blue). (B) Two sister neurons labeled with vsx1:GFP (left), filled with dye during whole-cell recording (right). One neuron co-labels with V2a marker chx10:Red (middle). Red arrow and blue arrow indicate sister V2a and presumed sister V2b, respectively. (C) Example traces during swim of vsx1 GFP+ sister neurons from V2a/b pair in voltage-clamp configuration. Asterisks denote detected excitatory postsynaptic current (EPSC) events. (D) Overlaid detected EPSC events recorded from sister V2a neuron (top) and simultaneously recorded signal in the sister V2b neuron (bottom). Most detected EPSCs in the V2a do not occur synchronously with EPSCs in the V2b neuron. (E) Overlaid detected EPSCs in V2b neuron (top) and simultaneously recorded signal in sister V2a neuron (bottom), also showing very few synchronous EPSCs. Colored traces represent averages of individual traces in gray. (F) Data from one example sister V2a/b pair showing the EPSC amplitude of detected events and the amplitude of the simultaneously recorded signal in the other neuron (Trig). Boxes depict medians, 25th and 75th percentiles. Whiskers denote 10th and 90th percentiles. Open circles depict EPSC values above and below the 10th and 90th percentiles. ***Wilcoxon signed-rank test (V2a – V2b simul.) p=1.8 × 10–206; (V2b – V2a simul.) p=6.7 × 10–15 paired t-test. (G) Summary data from all sister V2a/b pairs of recorded EPSC amplitudes and the EPSC-triggered simultaneously recorded signal in the other neuron. ***Wilcoxon signed-rank test, (V2a – V2b simul.) p=1.6 × 10–5; (V2b – V2a simul.) p=2.6 × 10–5 paired t-test, n = 13 pairs from 13 fish. (H) As in (F), for non-sister V2a/b paired recordings from the same spinal segment. ***Wilcoxon signed-rank test, (V2a – V2b simul.) p=1.6 × 10–5; (V2b – V2a simul.) p=1.3 × 10–4 paired t-test, n = 13 pairs from 13 fish. Source data for this figure are given in Figure 4—source data 1.
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