FIGURE

Fig. 2

ID

ZDB-FIG-221223-2

Publication

Lei et al., 2021 - Noncanonical protease-activated receptor 1 regulates lymphatic differentiation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

par1 is required for lymphatic differentiation in zebrafish embryos

(A) Immunostaining of prospero homeobox protein-1a (Prox1a) in Tg(fli1a:EGFP) siblings and par1 homozygous mutants at 54 hpf. White arrowheads indicate the presence of parachordal lymphangioblasts (PLs) sprouting in each somite; blue arrowheads with white dashed lines represent positive Prox1a staining in PLs. Scale bars: 100 μm.

(B) Prox1a-positive PLs in siblings (n = 17 embyos) and par1 homozygous mutants (n = 22 embryos) at 54 hpf; 7 somites/embryos were used for quantification. Table shows the number of prox1a-positive segment per 7 somites in each embryo.

(C) Relative mRNA expression of PROX1 and PAR1 in human dermal lymphatic endothelial cells (HDLECs) after ctr-siRNA (control) and PAR1-siRNA transfection.

(D) Western blot analysis of PROX1 and PAR1 expression upon PAR1 knockdown in HDLECs. In (C and D), values represent means ± SEMs. ∗p ≤ 0.01, ∗∗p ≤ 0.001 in the Student's t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience