Fig. 1
- ID
- ZDB-FIG-221223-1
- Publication
- Lei et al., 2021 - Noncanonical protease-activated receptor 1 regulates lymphatic differentiation in zebrafish
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par1 mutant zebrafish embryos show defective TD formation (A) WISH of par1 gene expression in zebrafish embryos at 26 hpf. The white arrowhead indicates the expression of the par1 gene in the PCV. (B) Brightfield lateral views of sibling and par1 homozygous mutant zebrafish embryos at 5 dpf. Scale bars: 1 mm. (C) Top row, schematic representation of the generated par1 zebrafish mutant; middle, results of sequencing for validating par1 mutants; bottom, DNA gel results for genotyping wildtype (+/+), heterozygous (+/−), and homozygous mutant (−/−) embryos. (D) Confocal images showing TD formation of sibling and par1 homozygous mutants using the Tg(fli1a:EGFP) line at 5 dpf. Blue arrowheads indicate the presence of TD formation in each somite; yellow asterisks represent the absence of TD formation in each somite; DA and PCV areas are denoted. Scale bars: 100 μm. (E) Confocal images showing the nuclear numbers of LECs in the TD tube in Tg(fli1aep:dsRed;fli1:nEGFP) siblings and par1 zebrafish mutants at 5 dpf. White circles indicate the presence of LECs nuclear numbers in the TD tube. Scale bars: 100 μm. (F) Percentage of somites lacking TDs in siblings (n = 40 embryos) and par1 homozygous mutants (n = 42 embryos); 6 somites/embryos were used for quantification. Right is the table showing the number of embryos with normal TD, partial TD, and without TD, respectively. (G) LECs nuclear number in the TD tube of siblings (n = 52 embryos) and par1 homozygous mutants (n = 33 embryos); 6 somites/embryos were used for quantification. In (F) and (G), values represent means ± SEMs. ∗p ≤ 0.001 in the Student's t test. See also Figure S1. |