Figure 5

Effects of the absence of pank2 on vasculature development. (A) Measurement of the venous return in embryos at 24 hpf; the blue line indicates the length of the caudal vein, and the red line is that of the tail. The measurement was expressed as the ratio between the two values (percentage). (B) The graph in the panel shows the mean values obtained in control and mutant embryos (Ctl = 51.6%; D4 = 47.9%). The experiment was repeated twice on 36 embryos/group. * p < 0.05, Student’s t-test. (C) Representative images of the phosphatase assay for vessel visualization on 72 hpf embryos. The red line decorates the dorsal aorta; the blue one is the caudal plexus. The numbers at the bottom of the images indicate the ratio between embryos with the observed phenotype and the total number of stained embryos. The experiment was performed 3 times on at least 60 embryos/group. Size bar = 500 µm.