FIGURE

Fig. 4

ID

ZDB-FIG-221109-33

Publication

Zhou et al., 2022 - Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction

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Fig. 4

a Representative Giemsa staining of PMNs and PBMCs separated from PBLs by density-gradient centrifugation and erythrocyte lysis (scale bar, 50 μm). b Quantitative RT-PCR of Dusp6 mRNA in PMNs and PBMCs from WT rats for sham-operated, MI 6 h, MI 24 h, and MI 72 h (performed in duplicates with n = 3 biological independent samples/group). c, d Western blot and corresponding quantitative analysis of DUSP6 in WT PMNs and PBMCs for sham-operated, MI 6 h, MI 24 h, and MI 72 h (n = 6 biological independent samples/group). GAPDH was used as an internal control. All blots within each individual panel were performed in parallel with the same samples. e Intracellular staining, flow cytometry and corresponding quantitative analysis of DUSP6 in HIS48⁺ and HIS48^– cells from PBLs of unoperated WT rats (n = 6 biological independent samples/group). All quantitative data shown in this figure are presented as mean values ± SD. Two-sided unpaired T-test was used to calculate the presented p-values. Source data of b–e are provided in a Source Data File. PMNs polymorphonuclear cells (peripheral neutrophils), PBMCs peripheral blood mononuclear cells, PBLs peripheral blood leukocytes, MFI, median fluorescence intensity.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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