FIGURE

FIGURE 6

ID
ZDB-FIG-221105-15
Publication
Chen et al., 2022 - TFG mutation induces haploinsufficiency and drives axonal Charcot-Marie-Tooth disease by causing neurite degeneration
Other Figures
(all 6)
All Figure Page
Back to All Figure Page
FIGURE 6

Wnt signaling is activated in response to TFG deficiency. (A) qRT‐PCR‐based validation of the key molecule in Wnt signaling in tfg morphants and control morphants at 3 dpf. (mean ± SD; two‐tailed unpaired t‐test, ***p < 0.001; n =  3). (B) qRT‐PCR‐based validation of the key molecule in Wnt signaling in primary cultured neurons on day 8 and 12 in culture after Tfg knockdown. (mean ± SD; two‐tailed unpaired t‐test; ns, nonsignificant; **p < 0.01; ***p < 0.001; n = 3). (C) Immunofluorescence staining of primary cultured neurons on day 8 after Tfg knockdown and pretreated with LGK974 for 24 h on day 4. Neurites were labeled with MAP2 (green) and the nucleus with DAPI (blue). Scale bars: 20 μm. (D) Quantification of the longest neurite length on day 8 in culture (mean ± SD; two‐tailed unpaired t‐test; ns, nonsignificant; ****p < .0001; n = 60).
Expression Data
Genes: (all 12)
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ CNS Neurosci Ther
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.