Fig. 1

Frequent mutations of SMARCAD1 in humans and zebrafish suggest it is a tumor suppressor. (A) Heat map of human chromosome 4. SAMRCAD1 is located on this chromosome. The locus of SMARCAD1 (green line) was found to be underrepresented in ~26% (16 out of 61) human MPNSTs.⁸ (B) Zebrafish smarcad1a is located on chromosome 8, which is underrepresented in about 70% (103 out of 147) of zebrafish MPNSTs. (C) Zebrafish smarcad1b is located on chromosome 10, which is overrepresented in about 60% (88 out of 147) of zebrafish MPNSTs. Samples are sorted top-to-bottom by decreasing deletion amplitude at the respective SAMRCAD1/smarcad1 locus, indicated by a green line. Blue and red bars on the right side of each panel indicate samples with SAMRCAD1/smarcad1 losses (blue) or gains (red). Color densities are corresponding to the degree of loss and gain as previously described.⁸ (D) SMARCAD1 mutation frequency of human cancers in TCGA database. (E) SMARCAD1 mutation types and positions found in human cancers in TCGA database. (F) SMARCAD1 expression level of 6 human MPNST cell lines (all NF1 mutant except STS26T) and HEI-193, immortalized human schwannoma