FIGURE

Figure 1.

ID

ZDB-FIG-221022-9

Publication

VanLeuven et al., 2018 - A PAGE screening approach for identifying CRISPR-Cas9-induced mutations in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 1.

Workflow overview of PCR and PAGE for screening CRISPR-Cas9-induced mutations in zebrafish.

F0-injected zebrafish are grown to adulthood and outcrossed to a wild-type zebrafish. 12 embryos from this outcross are sacrificed for genomic DNA extraction and PCR analysis of the region encompassing the target site. PCR products are directly run on a 15% polyacrylamide gel. This gel represents an outcross in which the F0-injected founder is carrying a single 10-bp deletion at gad2 exon 1 that is transmittable at a frequency of ∼33% to the F1 generation. The second pair of bands that are noted with an asterisk are heteroduplexes. These heteroduplexes are seen with all heterozygous samples for all alleles and are not a reflection of nonspecific primer binding.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biotechniques