FIGURE

FIGURE 2

ID

ZDB-FIG-221004-17

Publication

Lee et al., 2022 - A zebrafish model of congenital nephrotic syndrome of the Finnish type

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FIGURE 2

The expression analyses of nephrin and podocin in nphs1 mutants. Whole-mount in situ hybridization for nephrin (A–B) and podocin (C–D) at 54 hpf (E–F) Quantitative PCR analyses of mRNA levels of nphs1 and nphs2 show no statistically significant difference between nphs1 heterozygotes (+/-) and nphs1 homozygous mutants (-/-) at 7 dpf for both 5bp deletion (d5) and 28bp deletion (d28) mutant alleles. The expression level is normalized to that of eflα. The error bars show the standard error of the mean (SEM). (G–H) Confocal images of Immunofluorescence staining for nephrin (G, red) and podocin (H, red) at 10 dpf. Podocytes are marked by GFP expression (green) by the Tg (pod:GFP) transgene. No nephrin expression is detected in Tg (pod:GFP); nphs1-/- at 10 dpf, while podocin is present with a grainy pattern.

Expression Data

Genes:	EGFP nphs1 nphs2
Antibodies:	Ab3-nphs1 Ab4-nphs2
Fish:	AB nphs1^{mss102/mss102} (AB) nphs1^{mss103/mss103} (AB) nphs1^mss102/+ (AB) nphs1^mss103/+ (AB) nphs1^mss102/+; zf238Tg nphs1^{mss102/mss102}; zf238Tg nphs1^mss103/+; zf238Tg nphs1^{mss103/mss103}; zf238Tg (all 9)
Anatomical Terms:	pronephric podocyte whole organism
Stage Range:	Long-pec to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nphs1^{mss102/mss102}; zf238Tg nphs1^{mss103/mss103}; zf238Tg
Observed In:	pronephric podocyte
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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