Fig. 5

Knockout of sema4C caused both aberrant phenotypes in PMNs and ISVs. (A). The targeting site of the gRNA on the sema4C gene; (B). The measure of cutting efficiency using genomic DNAs of injected embryonic (n = 10); (C). Confocal imaging analysis of PMNs and ISVs in control and the F0 generation of the injected Tg(mnx1:EGFP::kdrl:ras-mCherry) zebrafish at 48 hpf, blue arrowheads indicate aberrant PMN and ISV; (D). The statistical analysis of the proportion of abnormal axonal trajectories of Caps (*** p = 0.0006) and short Caps (*** p = 0.0006) in the different groups at 48 hpf. Mann–Whitney test; (E). The statistical analysis of the proportion of vessels that extend across different segments (*** p = 0.0006) and the proportion of proliferated vessels (*** p = 0.006) in the different groups at 48 hpf. Mann–Whitney test.