Locomotor activity of 6 dpf zebrafish and Dc larvae in the light-dark test. (A) The light-dark test consists of a habituation (20 min; orange), swimming (20 min; blue) and two alternating dark (10 min each; gray) and light (10 min each; yellow) phases. The average locomotor activity ± the SEM (shaded) per second is shown for zebrafish AB wildtype (green; n = 60), crystal (blue; n = 60), and Dc (red; n = 40) larvae; color-coded arrowheads highlight the increases in locomotor activity 1 s after the illumination switch. (B,C) Violin plots of the velocity during movement (B) and percentage of time spent moving (C) of zebrafish AB wildtype, crystal, and Dc during the habituation (orange) and swimming phase (blue); the mean is indicated by a dotted black line. (D,E) Violin plots of the velocity during movement (D) and percentage of time spent moving (E) of zebrafish AB, crystal, and Dc during the light (yellow) and dark phases (gray); the mean is indicated by a dotted black line. Two-way ANOVA followed by Šídák’s or Tukey’s multiple comparisons test was used to analyze differences in velocity or movement between phases of the light-dark test in and between AB, crystal, and Dc; p > 0.05 is abbreviated as not significant (n.s.).
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