ZFIN ID: ZDB-FIG-220803-19
Kamel et al., 2022 - The zebrafish cohesin protein Sgo1 is required for cardiac function and eye development. Developmental dynamics : an official publication of the American Association of Anatomists   251(8):1357-1367 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 4-cell to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Days 14-20

Fig. 1

The generation of sgo1 mutant in zebrafish. (A) sgo1 transcript in zebrafish (Danio rerio) displaying the exons and intron regions. Human (Homo sapiens) SGO1 protein, containing 527 amino acids, and binding regions: protein phosphatase 2 (PP2A), chromosome passenger complex (CPC), heterochromatin protein 1 (HP1), and histone H2A (H2A). Blue arrow shows the location of the generated mutant in exon 5. (B) RT-PCR for sgo1 and ef1a (control) at various developmental stages. (C) CRISPR/Cas9 design for the introduction of a loss-of-function mutation in sgo1. The mutated sequence shows a 17 bp deletion in exon 5, resulting in an early stop in the mutated sequence and truncation of the protein (not shown). (D) Top and side images of 5 dpf larvae of sgo1−/−, sgo1+/− and their wild-type siblings. (E) Kaplan–Meier curve, displaying the survival of sgo1 −/−, sgo1+/− and their wild-type siblings during the first 120 dpf of development (sgo1+/+ n = 14, sgo1+/− n = 30, sgo1−/− n = 16). (F) Levels of sgo1 expression in sgo1 mutant embryos (n = 5) in comparison with wild type siblings (n = 5). Statistics: (E) Log-rank (Mantel–Cox) test, mean ± SEM; (F) Two-tailed nonpaired Student's t-test, mean ± SD; P ≤ .0001. Scale bars is at 200 μm. bp: base pairs, PAM: protospacer adjacent motif, dpf: days post fertilization

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
sgo1 TL standard conditions 4-cell whole organism RTPCR
Shield whole organism RTPCR
Bud Not Detected whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Day 5 whole organism RTPCR
sgo1hu13334/hu13334 (TL) standard conditions Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sgo1hu13334/+ (TL) standard conditions Protruding-mouth whole organism morphology, normal
Protruding-mouth whole organism viable, normal
Day 5 swim bladder inflated, normal
Day 5 whole organism viability, normal
Day 5 whole organism viable, normal
Days 14-20 whole organism viability, normal
Days 14-20 whole organism viable, normal
sgo1hu13334/hu13334 (TL) standard conditions Protruding-mouth whole organism morphology, normal
Protruding-mouth whole organism viable, normal
Day 5 swim bladder uninflated, abnormal
Day 5 whole organism sgo1 expression decreased amount, abnormal
Day 5 whole organism viability, normal
Day 5 whole organism viable, normal
Days 14-20 whole organism dead, abnormal
Days 14-20 whole organism viability, abnormal
Acknowledgments:
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