FIGURE

Figure 4

ID

ZDB-FIG-220709-4

Publication

Taznin et al., 2022 - Cytokine Receptor-Like Factor 3 (CRLF3) Contributes to Early Zebrafish Hematopoiesis

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Figure 4

Mutation of crlf3 impacts early definitive hematopoiesis. (A–X). Homozygous wild-type crlf3^wt/wt (wt/wt) and crlf3^mdu14/mdu14 (mdu14/mdu14) embryos were subjected to WISH with cmyb(A, B) at 4 dpf, and lcp1(D, E), mpo(G, H), hbbe(J, K), ikzf1(M–N), rag1(P, Q) and tcra(S, T) at 5 dpf (scale bar = 200 μm), or underwent blood analysis (V–W) at 5 dpf (scale bar = 10 μm). Individual embryos were assessed for the area of staining of cmyb CHD region (dotted boxes in panels A and B) (C), hbbe(L), ikzf1(O), rag1(R) and tcra(U), as well as the number of lcp1⁺(F) and mpo⁺(L) cells or for blood differential counts (X), with mean and SEM in red and statistical significance indicated (***p < 0.001, **p < 0.01, *p < 0.05, ns, not significant). Welch’s correction was used for panels (C, L).

Expression Data

Genes:	ikzf1 lcp1 mpx myb rag1 trac
Fish:	WT crlf3^mdu14/mdu14
Anatomical Terms:	caudal hematopoietic tissue hematopoietic stem cell monocyte neutrophil thymus thymus lymphoid progenitor cell
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	crlf3^mdu14/mdu14
Observed In:	caudal hematopoietic tissue hematopoietic stem cell definitive hemopoiesis monocyte neutrophil thymus
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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