FIGURE

Fig. 5

ID

ZDB-FIG-220512-32

Publication

Capon et al., 2022 - Endocardial identity is established during early somitogenesis by Bmp signalling acting upstream of npas4l and etv2

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Fig. 5

Bmp signalling is active in developing endocardial cells. (A,B) Immunofluorescence staining for pErk1/2 (A) in Gt(endocard:egfp) and Tg(fli1a:egfp) embryos at 15 s shows minimal pErk1/2 signal in endocardial cells and high activity in the adjacent vascular endothelium. By contrast, pSmad1/5/8 (B) in Gt(endocard:egfp) and Tg(fli1a:egfp) embryos at 15 s shows high pSmad1/5/8 activity in developing endocardial cells but minimal activity in adjacent vasculature. Quantification of pErk1/2 area and intensity or pSMAD cell number in endocardium or adjacent vasculature is depicted in neighbouring graphs. Dorsal views are shown with anterior to the top in all images. Scale bars: 50 μm. Data are mean±s.d. P-values are present in graphs (determined by an unpaired two-tailed t-test).

Expression Data

Antibodies:	Ab9-mapk Ab13-smad
Fish:	y1Tg/y1Tg map3k22^{nksagff27cGt/nksagff27cGt}
Anatomical Terms:	endocardial precursor vascular endothelium
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg/y1Tg map3k22^{nksagff27cGt/nksagff27cGt}
Observed In:	endocardial precursor vascular endothelium
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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