scRNA-seq dataset of zebrafish organogenesis and connexin expression. ai) Clustered cell types, where each dot represents a single cell and each color represents a set of transcriptionally related cells. aii) The age of animals from which cells were dissociated denoted by color—1 dpf cells are blue, 2 dpf cells are orange, and 5 dpf cells yellow. bi–biii) Expression of well-studied connexins in the dataset, where gray represents low expression and red represents the highest level of expression. bi) gjc4b/Cx43.4 is expressed broadly across the dataset. bii) gja1b/Cx43 is expressed in a large number of clusters, with notable patterns in liver, endothelial, macrophage, neural crest, spleen, retina, kidney, epiphysis, osteoblast, mesoderm, tailbud, pigment cells and lens clusters. biii) gja8b/Cx44.1 is expressed in lens clusters. ci) Broadly expressed connexins, gja1b/Cx43 and gjc4b/Cx43.4 and (cii) the remaining connexin family shown for each sampled time point. Here, all cells from the corresponding age are pooled and the percent of cells expressing a given connexin are represented through dot size while the relative expression level is denoted through color intensity.
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