FIGURE

FIGURE 3

ID

ZDB-FIG-220413-18

Publication

Wei et al., 2022 - Dual-Specificity Phosphatase 14 Regulates Zebrafish Hair Cell Formation Through Activation of p38 Signaling Pathway

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FIGURE 3

Knockdown dusp14 expression by morpholino decreases the number of hair cell and neuromasts in zebrafish. (A) Schematic diagram of zebrafish neuromast and inner ear hair cell. (B) Confocal imaging analysis of crista hair cells in the otic vesicle of control and dusp14 deficiency zebrafish at 72 hpf. (B′) The statistical analysis of the numbers of inner ear crista hair cells in the control and dusp14 morphants at 72 hpf. (B′′) The statistical analysis of the numbers of hair cells in the remaining neuromast L3 in the control and dusp14 morphants at 72 hpf. (C) The imaging analysis of control and dusp14 morphants at 72 hpf in fluorescent field. Scale bar = 500 μm. (C′) Quantification of the number of unilateral hair cell clusters of control and dusp14 morphants at 72 hpf. (D)in situ hybridization of the eya1 gene specifically expressed in the neuromasts showed that the number of neuromasts in dusp14-MO zebrafish was decreased. (D′) statistics results of D. Each bar represents the mean ± SEM. Values with *, ***, and **** above the bars are significantly different (P < 0.05, P < 0.001, and P < 0.0001, respectively).

Expression Data

Gene:	eya1
Fish:	AB AB + MO1-dusp14
Knockdown Reagent:	MO1-dusp14
Anatomical Term:	posterior lateral line neuromast
Stage:	Protruding-mouth

Expression Detail

Gene	Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
eya1		AB	standard conditions	Protruding-mouth		posterior lateral line neuromast	ISH
eya1		AB + MO1-dusp14	standard conditions	Protruding-mouth		posterior lateral line neuromast	ISH

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Antibody Labeling

Phenotype Data

Fish:	AB + MO1-dusp14
Knockdown Reagent:	MO1-dusp14
Observed In:	auditory receptor cell neuromast hair cell posterior lateral line neuromast
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.