FIGURE

Fig 1

ID

ZDB-FIG-220325-19

Publication

Lu et al., 2022 - mTOR activity is essential for retinal pigment epithelium regeneration in zebrafish

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Fig 1

The mTOR pathway is activated in the RPE after ablation.

(A) Schematic of the experimental paradigm showing the time points for MTZ ablation and sample collection on transgenic larvae (rpe65a:nfsB-eGFP). (B-H) Immunofluorescent images of p-S6 staining on transverse cryosections from unablated (MTZ⁻) and (I-O) ablated (MTZ⁺) larvae from 3hpi to 4dpi. White arrows indicate the p-S6⁺ eGFP^- cells in the RPE layer. White arrowheads indicate p-S6⁺ cells in the retinae. (J’-J”‘) High-magnification images showing the colocalization of p-S6 and eGFP at 6hpi, 12hpi (K’-K”‘) and 1dpi (L’-L”‘). Nuclei (white), eGFP (green), p-S6 (magenta). (P) Quantification of the p-S6⁺ area in the RPE layer revealed that expression was low in the RPE of unablated larvae but increased significantly in ablated larvae from 3hpi to 3dpi. p-values: ** ≤ 0.01, *** ≤ 0.001. Statistical information can be found in S9 Table. Dorsal is up and distal is left. Scale bar = 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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