Figure 3

miR-99 overexpression suppresses the expression of roraa in zebrafish neutrophils. (A) Volcano blot of DEGs with significant expression changes in miR-99 expressing neutrophils. Blue: down-regulated differentially expressed genes (DEGs); Magenta: up-regulated DEGs. (B) Significantly altered pathways in the down-regulated DEGs. (C) Alignment of miR-99-1-5p and predicted miR-99 binding sites in zebrafish roraa 3’UTR. (D) Transcript level quantification of predicted downregulated genes in neutrophils sorted from the vector or miR-99 zebrafish line. Results from three independent experiments are normalized to rpl32 and presented as mean. Holm-Sidak test. (E) Suppression of Renilla luciferase expression via binding to zebrafish roraa 3’UTRs by miR-99. Results from three independent experiments are normalized to firefly luciferase and presented as mean ± s.d., Kruskal–Wallis test.