FIGURE

Fig. 5

ID

ZDB-FIG-220131-73

Publication

Hong et al., 2021 - IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

<a Schematic illustration of a driver under the control of elavl3 promoter and respective effectors harboring different numbers of QUAS. The driver constructs were equipped with 8.7 kb of elavl3 promoter instead of ubb promoter. Teb (50 µM) was administered for 48 h before imaging at 5 dpf. b Genetic cross of F1 Tg(elavl3:QFDBD-2xAD*-VP16-EcR) with indicated F2 effector lines. The siblings were exposed to Teb to induce ZsGreen transgene expression. Note that the intensity of ZsGreen reporter fluorescence in the pan-neuronal tissues driven by elavl3 promoter was gradually augmented in the order of the increased number of QUAS repeats. c Genetic cross of F1 Tg(elavl3:QFDBD-2xAD*-VP16*) with indicated F2 effector lines. The intensity of ZsGreen fluorescence among the siblings was most prominent with an effector harboring a 13xQUAS transgene. Abbreviations: Teb; tebufenozide. Scale bar: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Commun Biol