FIGURE

Fig. 1

ID

ZDB-FIG-211230-30

Publication

Fadul et al., 2021 - KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion

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Fig. 1

KRas^V12 induces formation of epidermal cell masses and basal extrusion at distinct sites.

a 26 hpf wild-type zebrafish embryos expressing krt4:EGFP-CAAX or dt-KRas^V12, masses indicated by black arrowheads and inset. b Mean of cell masses per embryo ± SEM, P < 0.0001 by a Mann–Whitney test, n = embryos. c Map of where cell masses occur, as observed at 28 hpf. d Stills from time-lapse imaging (hh:mm) of periderm:Lifeact-EGFP mosaically expressing EGFP-CAAX or EGFP-dt-KRas^V12, showing hallmarks of apical and basal extrusion, with XZ sections beneath showing constriction at the base or apex, respectively (white arrows delimit ring constricting). e Number of apical and basal extrusions in EGFP-CAAX or dt-KRas^V12 embryos. Data are mean ± SEM, **P < 0.02 by a Mann–Whitney test for apical and basal extrusions pooled together. f Maps indicating where apical and basal extrusions occur in 28-46 hpf embryos and 55–73 hpf embryos, where different colours represent different fish analyzed, n. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.