Fig. 2.
- ID
- ZDB-FIG-211216-237
- Publication
- Miles et al., 2021 - Usher syndrome Type 1-associated gene, pcdh15b, is required for photoreceptor structural integrity in zebrafish
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Cone and rod photoreceptors show reductions in OS number and length that are not attributed to cell death or loss. (A) Staining and quantification of the cell death marker, cleaved (activated) caspase-3, at 10 dpf in the ONL per section (+/+, 12 eyes) (Δ7, 12 eyes). (B) Quantification of the number of pyknotic nuclei in the ONL. (C) Photoreceptor nuclei (Hoechst) density/μm2 in the ONL at 10 dpf, measured from an average of 20 µm-thick sections. For B and C: +/+, 12 eyes; Δ7, 11 eyes; +/+, 8 eyes; ins17, 7 eyes. (D) Representative images of OS markers for cones (PNA lectin, red) and rods (WGA lectin, green) at 10 dpf and 15 dpf. (E) TEM images of the central retina of wild-type siblings (+/+) and Δ7 and ins17 mutants at 10 dpf. Magenta represents individual photoreceptor; green represents detached OS. (F,G) Quantification of the number of OSs in the inner row at 10 dpf (F), 5 dpf (G, left) and 15 dpf (G, right). (H) Quantification of the average length of all outer segments in the retina per section in wild-type siblings and Δ7 and ins17 mutants at 5 dpf and 10 dpf. Statistical analyses in A-C and F-H were performed using Student's t-tests (unpaired, two-tailed). n.s., not significant; *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001. Number of eyes analysed for TEM data in E-G: 5 dpf: +/+, 10 eyes; Δ7/Δ7, 10 eyes; 10 dpf: +/+, 9 eyes; Δ7/Δ7, 12 eyes; +/+, 8 eyes; ins17/ins17, 7 eyes; 15 dpf: +/+, 8 eyes; Δ7/Δ7, 8 eyes. At least n=5 individuals were assessed for each immunostained marker. IS, inner segment; ONL, outer nuclear layer; OS, outer segment. |
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| Stage: | Days 7-13 |