Fig. 1

NXT treatment restored TFD-induced cardiomyopathy in zebrafish embryos. A Lateral view of four groups of zebrafish embryos at 4 dpf. TFD treated group showing pericardial edema (red dashed line) and slowed blood flow (red arrows). B Blood flow motion ratios of four groups of zebrafish embryos based on pixel density changes in RBCs. C Optimal recovery concentration of NXT. D Pericardial area of four groups of zebrafish larvae (n = 15 embryos/group). E Heart rates of four groups of zebrafish larvae (n = 15 embryos/group) F SV-BA distance of the four groups of zebrafish larvae (n = 15 embryos/group). G The heart morphology of four groups embryos at 96hpf delineated by Tg(cmlc2:GFP) (ventral view). Note the enlarged ventricles in the TFD treatment group (V ventricle; A atria). Data are represented mean ± standard deviation (SD) from three independent experiments, ^##p < 0.01, ^###p < 0.001 vs control group; **p < 0.01, ***p < 0.001 vs TFD-induced group (Student’s t test)