FIGURE

Fig. s4

ID
ZDB-FIG-211105-23
Publication
Eom et al., 2021 - Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes
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Fig. s4

jam3b:Jam3b-mCherry expression during post-embryonic development. (A) Basal epidermal cells of the skin. (B) Superimposed brightfield and fluorescence of image of Jam3b-mCherry expression in lens of a juvenile fish. (C) Melanophores of the stripe. In brightfield (captured by photomultiplier tube; PMT) melanin is evident, contracted by epinephrine to centers of melanophores. Reflecting platelets of sparsely arranged stripe iridophores are also evident (e.g., arrow). Melanophores express mitfa:EGFP, with jam3b:Jam3b-mCherry accumulations at cell peripheries. In contrast to melanophores beneath them, stripe iridophores did not have detectable accumulation of Jam3b-mCherry. Asterisk, lipid droplet in underlying adipocyte. (D) In the interstripe, reflecting platelets of densely packed iridophores are apparent. In brightfield-PMT, accumulations of carotenoid pigments within xanthophores appear black (dashed circles). Iridophores and reflecting platelets are labeled with pnp4a:EGFP and Jam3b-mCherry is evident where cells contact one another. Scale bars, 20 μM (A,C,D), 500 μM (B).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 476, Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M., Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes, 314-327, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.