FIGURE

Fig. 5

ID
ZDB-FIG-211105-18
Publication
Eom et al., 2021 - Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Jam3b promotes aggregation in vitro and an adherent morphology in vivo. (A) S2 cells transfected with constructs to express actin-GFP (control), wild-type Jam3b-mCherry or A96E mutant Jam3b-mCherry. Aggregates of cells expressing wild-type Jam3b formed within 60 ​min of rotary culture. (B) Quantification of transfected cells found within clusters relative to the total numbers of transfected cells in 3 replicate cultures. ∗∗∗, Tukey-Kramer post-hoc comparisons of means P ​< ​0.0001 (overall ANOVA, F2,6 ​= ​82.6, P ​< ​0.0001; data were arcsine-transformed for analysis to correct heterogeneity of variance among groups). (C) Membrane-targeted mCherry revealed well-spread morphologies of wild-type melanophores but more dendritic morphologies of jam3butr8e1 and igsf11utr15e1 mutant melanophores in vivo. (D) Melanophores of jam3bvp8re2 mutants were also dendritic but adopted a more spread appearance when expressing wild-type Jam3b transgenically. Scale bars 20 ​μM (D, for C and D).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 476, Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M., Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes, 314-327, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.