Core behavioral features of ASD-like displayed in shank3-deficient zebrafish. (A,B) The shoaling test showed significantly increased average inter-fish distance of adult male shank3-deficient zebrafish (3.5 mpf). WT, N = 18; shank3a– /–, N = 14; shank3b– /–, N = 13; shank3ab– /– zebrafish, N = 21. (C,D) The social preference test showed distance ratio in the conspecific sector were significantly reduced in shank3-deficient zebrafish compared to WT adult male zebrafish (3.5 mpf). WT, N = 16; shank3a– /–, N = 14; shank3b– /–, N = 16; shank3ab– /– zebrafish, N = 15. (E,F) The Kin recognition and preference test showed significantly reduced ratio of Kin zone entering in shank3-deficient zebrafish compared to WT adult male zebrafish (3.5 mpf). WT, N = 9; shank3a– /–, N = 10; shank3b– /–, N = 12; shank3ab– /– zebrafish, N = 16. (G) Representative trace of different types of stereotyped behaviors of shank3-deficient adult male zebrafish (3.5 mpf). (H)shank3-deficient zebrafish had a significantly higher proportion of stereotyped movements than WT zebrafish. WT, N = 12; shank3a– /–, N = 14; shank3b– /–, N = 14; shank3ab– /– zebrafish, N = 16. Data are presented as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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