FIGURE 1

3d-printed tools for imaging on upright and inverted microscopes. The left column shows schematics of the different imaging modalities. The middle column shows the 3d-printed mounting tools. The right column shows examples of embryos mounted using the tools. (A) An iterative design strategy was used to optimize pins on 3d-printed mounting tools for different imaging modalities on upright (left) and inverted (right) microscopes. (B) Tool to mount embryos in a holder used for imaging on a lattice light-sheet microscope (LLSM). (C) Tool to mount embryos in a Petri dish for imaging on an upright microscope, such as a diSPIM. The pins sit on a plate with a slight offset from the actual tool block. This generates a shallow pool on the imaging plate after removal of the medium, allowing direct access for water dipping lenses to image the mounted embryos. (D) Tool to mount embryos in a glass-bottom dish for imaging on an inverted microscope, e.g., by spinning disk microscopy. Similar to the tool for Petri dishes, this tool has a feature to produce shallow wells for mounting, a trench that runs around the wells.