Figure 9—figure supplement 2.

The barrier function of the <italic toggle='yes'>hai1a</italic> epidermis is not grossly compromised.

(A–D′) Lateral confocal images of the tails (AB′; D, D′) and trunk (C, C′) of 36hpf WT (AA′; DD′) and hai1ahi2217 embryos (B–C′) immersed in 2.5 mg/ml of 3–5 kDa fluorescein dextran for 30 min before brief destaining. Fluorescent images (A–D) are also shown overlaying DIC images (A′–D′). (E, F) Lateral DIC images of the tails (E, F, H) and trunk (G) of 36hpf WT (E, H) and hai1ahi2217 embryos (F, G) immersed in 0.075% of methylene blue for 30 min before brief destaining. Tails of WT embryos in (H) were wounded by scalpel cut before immersion. Scale bars: (D′, H) = 50 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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