FIGURE

Figure 4

ID
ZDB-FIG-210708-26
Publication
Mikdache et al., 2021 - Rgs4 is a regulator of mTOR activity required for motoneuron axon outgrowth and neuronal development in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Figure 4

MZrgs4 embryos show a specific decrease in the number of HuC+ cells and a significant increase in the number of PH3+ cells in the spinal cord. (A) Lateral views of spinal cord Z projections (around 15 μm) following whole-mount immunostaining for HuC (left) and counterstained with DAPI (middle) with merged still images (right) in WT (top) and MZrgs4 larvae (bottom) at 48 hpf. Scale bar = 20 μm. (B) Quantification of the number of HuC+ and HuC− cells within the spinal cord in WT (average of 101.2 ± 3.12 for HuC+ ; average of 93.83 ± 7.36 for HuC−, n = 6) and MZrgs4 larvae (average of 51.86 ± 5.25 for HuC+ ; average of 84.71 ± 4.39 for HuC−, n = 7) at 35 hpf (left) and at 48 hpf in WT (right) (average of 166.7 ± 7.04 for HuC+ ; average of 146.2 ± 8.28 for HuC−, n = 6) and MZrgs4 larvae (average of 130.3 ± 3.7 for HuC+ ; average of 153.1 ± 3.99 for HuC−, n = 7). (C) Lateral views of spinal cord Z projections (around 30 μm for PH3) following whole-mount immunostaining for PH3 (left) and HuC (middle) with merged still images (right) in WT (top) and MZrgs4 larvae (bottom) at 48 hpf. (D) Quantification of the number of PH3+ cells within the spinal cord in WT (average of 14.29 ± 1.82 at 35 hpf, n = 7; average of 4 ± 1.08 at 48 hpf, n = 9) and MZrgs4 larvae (average of 19.56 ± 1.10 at 35 hpf, n = 9; average of 12.44 ± 1.51 at 48 hpf, n = 9). (E) Dorsal views of head Z projections (around 400 μm) following HuC whole-mount immunostaining in WT and MZrgs4 larvae at 48 hpf. Dashed lines represent the nervous system. Scale bar = 50 μm. (F) Quantification of the head area in WT (average of 180,194 ± 3289, n = 9) and MZrgs4 larvae (average of 153,316 ± 5151, n = 9) at 48 hpf. (G) Quantification of the head perimeter in WT (average of 1677 ± 31.90, n = 9) and MZrgs4 larvae (average of 1564 ± 30.24, n = 9) at 48 hpf.

Expression Data
Antibodies:
Fish:
Anatomical Terms:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Prim-15 to Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.